Catalog

11200

Mini Sizer 50bp DNA Ladder

( 100 loads )
Norgen Biotek

172.06$

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Additional description

MiniSizer 50 bp DNA Ladder (Cat# 11200 )

DNA Ladder Promotion - Order three or more Norgen DNA Ladders at a special price.


Ideal for quick assessment of PCR products

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise
  • fourteen discrete fragments ranging from 25 bp to 650 bp in 50 bp increments
  • double intensity reference bands at 200 bp and 400 bp

Product Description

The Norgen MiniSizer 50 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains fourteen discrete fragments ranging from 25 bp to 650 bp in 50 bp increments with double intensity reference bands at 200 bp and 400 bp. The MiniSizer is ideal for PCR product size confirmation.

Contents:

1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

FEATURES AND BENEFITS

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise

Specifications

Norgen’s DNA Ladders/Markers have been genetically engineered so that the bands are of precise and discrete sizes. Well-spaced bands and higher-intensity reference bands facilitate visual analysis of the gel. These ladders are ideal for quantification due to the precise known ng amounts of each band.Our ladders are ideal for a wide range of applications including size and mass determination of digested DNA and PCR products.

Figure 1: MiniSizer 50 bp DNALadder

APPLICATIONS

MiniSizer50 bp DNALadder (Cat# 11200) - 100 loads

Ladder Properties:
• Fourteen discrete bands, ranging from 50bp to 650bp.
• Higher intensity bands at 200bpand 400bp for easy reference.

Fragment
Size (bp)
Mass (ng)
1
650
61
2
600
46
3
550
36
4
500
30
5
450
35
6
400
85
7
350
27
8
300
25
9
250
16
10
200
54
11
150
33
12 100 21
13 50 17
14 25 14

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:

Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.