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Catalog

11300

PCR Ranger 100bp DNA Ladder

( 100 loads )
Norgen Biotek

153.96$

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Additional description

PCR Ranger 100 bp DNA Ladder (Cat# 11300 )

DNA Ladder Promotion - Order three or more Norgen DNA Ladders at a special price.


Ideal for assessment of a range of PCR product sizes

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise
  • Eleven discrete fragments ranging from 50bp to 1000bp
  • Higher intensity reference band at 500bp.

Product Description

The Norgen PCR Ranger 100bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCR Ranger DNA Ladder contains eleven discrete fragments ranging from 50bp to 1000bp with a higher intensity reference band at 500bp. This Ladder is ideal for assessment of a range of PCR product sizes.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

FEATURES AND BENEFITS

  • Eleven discrete fragments from 50bp to 1000bp
  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise

Specifications

Norgen’s DNA Ladders/Markers have been genetically engineered so that the bands are of precise and discrete sizes. Well-spaced bands and higher-intensity reference bands facilitate visual analysis of the gel. These ladders are ideal for quantification due to the precise known ng amounts of each band.Our ladders are ideal for a wide range of applications including size and mass determination of digested DNA and PCR products.

Figure 1. PCRRanger 100 bp DNALadder.

APPLICATIONS

PCRRanger 100 bp DNALadder (Cat# 11300) - 100 loads

Ladder Properties:
• Eleven discrete bands, ranging from 50bp to 1,000bp.
• Higher intensity band at 500bp for easy reference.

Fragment
Size (bp)
Mass (ng)
1
1000
91
2
900
66
3
800
61
4
700
51
5
600
43
6
500
70
7
400
28
8
300
23
9
200
30
10
100
22
11
50
15

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.