Catalog

11500

LowRanger 100bp DNA Ladder

( 100 loads )
Norgen Biotek

153.96$

Add this item to your cart
retour

Additional description

LowRanger 100 bp DNA Ladder (Cat# 11500 )

DNA Ladder Promotion - Order three or more Norgen DNA Ladders at a special price.


Ideal for fast running times and accurate visual determination

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise
  • Eleven discrete fragments ranging from 100bp to 2000bp in 100bp increments
  • Higher intensity reference band at 500bp

Product Description

The Norgen LowRanger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our LowRanger contains eleven discrete fragments ranging from 100bp to 2000bp in 100bp increments with a higher intensity reference band at 500bp. This Ladder is ideal for fast running times and accurate visual determination.

Contents:


1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

FEATURES AND BENEFITS

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise



Specifications

Norgen’s DNA Ladders/Markers have been genetically engineered so that the bands are of precise and discrete sizes. Well-spaced bands and higher-intensity reference bands facilitate visual analysis of the gel. These ladders are ideal for quantification due to the precise known ng amounts of each band.Our ladders are ideal for a wide range of applications including size and mass determination of digested DNA and PCR products.

Figure 1: LowRanger 100 bp DNALadder

APPLICATIONS

LowRanger 100bpDNALadder (Cat# 11500) - 100 loads

Ladder Properties:
• Eleven discrete bands, ranging from 100bp to 2,000bp.
• Higher intensity band at 500bp for easy reference.

Fragment
Size (bp)
Mass (ng)
1
2000
110
2
1500
83
3
1000
55
4
800
44
5
700
39
6
600
33
7
500
55
8
400
22
9
300
17
10
200
22
11
100
22

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.