Catalog

11600

Clone Sizer 100bp DNA Ladder

( 100 loads )
Norgen Biotek

153.96$

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Additional description

CloneSizer 100 bp DNA Ladder (Cat# 11600 )

DNA Ladder Promotion - Order three or more Norgen DNA Ladders at a special price.


Includes a 2686bp (pUC19) reference band for easy clone identification

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise
  • Fourteen discrete fragments ranging from 100bp to 2000bp in 100bp increments
  • Higher intensity reference bands at 500bp and 1000bp
  • 2686bp (pUC19) reference band for easy clone identification

Product Description

The CloneSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our CloneSizer contains fourteen discrete fragments ranging from 100bp to 2000bp in 100bp increments, double intensity reference bands at 500 and 1000bp and an additional 2686bp (pUC19) reference band for easy clone identification.

Contents:


1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

FEATURES AND BENEFITS

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise

Specifications

Norgen’s DNA Ladders/Markers have been genetically engineered so that the bands are of precise and discrete sizes. Well-spaced bands and higher-intensity reference bands facilitate visual analysis of the gel. These ladders are ideal for quantification due to the precise known ng amounts of each band.Our ladders are ideal for a wide range of applications including size and mass determination of digested DNA and PCR products.

Figure 1: CloneSizer 100 bp DNALadder

APPLICATIONS

CloneSizer 100 bpDNALadder (Cat# 11600) - 100 loads

Ladder Properties:
  • Fourteen discrete fragments ranging from 100bp to 2000bp in 100bp increments
  • Higher intensity reference bands at 500bp and 1000bp
  • 2686bp (pUC19) reference band for easy clone identification

Fragment
Size (bp)
Mass (ng)
1
2686

72

2
2000
53
3
1500
41
4
1200
42
5
1000
56
6
900
30
7
800
29
8
700
25
9
600
25
10
500
52
11
400
19
12 300

20

13 200

19

14 100

17

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:

Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.