Catalog

11800

FullRanger 100bp DNA Ladder

( 100 loads )
Norgen Biotek

172.06$

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Additional description

FullRanger 100 bp DNA Ladder (Cat# 11800 )

DNA Ladder Promotion - Order three or more Norgen DNA Ladders at a special price.


  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise
  • Sixteen discrete fragments ranging from 100bp to 5000bp
  • Higher intensity reference bands at 500bp and 1000bp

Product Description

The Norgen FullRanger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our FullRanger contains sixteen discrete fragments ranging from 100bp to 5000bp with higher intensity reference bands at 500bp and 1000bp. This Ladder is ideal for accurate visual determination in both large and small cloning applications.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

FEATURES AND BENEFITS

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise



Specifications

Norgen’s DNA Ladders/Markers have been genetically engineered so that the bands are of precise and discrete sizes. Well-spaced bands and higher-intensity reference bands facilitate visual analysis of the gel. These ladders are ideal for quantification due to the precise known ng amounts of each band.Our ladders are ideal for a wide range of applications including size and mass determination of digested DNA and PCR products.

Figure 1: FullRanger 100 bp DNALadder

APPLICATIONS

FullRanger 100 bpDNALadder (Cat# 11800) - 100 loads

Ladder Properties:
•Sixteen discrete bands, ranging from 100bp to 5,000bp.
• Higher intensity reference bands at 500bp and 1000bp

Fragment
Size (bp)
Mass (ng)
1
5000
55
2
4000
44
3
3000
33
4
2500
28
5
2000
22
6
1500
17
7
1000
50
8
900
35
9
800
31
10
700
43
11
600
24
12 500

56

13 400

16

14 300

22

15 200

15

16 100

11

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:

Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.