Warning: date(): It is not safe to rely on the system's timezone settings. You are *required* to use the date.timezone setting or the date_default_timezone_set() function. In case you used any of those methods and you are still getting this warning, you most likely misspelled the timezone identifier. We selected the timezone 'UTC' for now, but please set date.timezone to select your timezone. in /home/teknisc/public_html/__biobar.ca/content/catalog.php on line 49

Warning: date(): It is not safe to rely on the system's timezone settings. You are *required* to use the date.timezone setting or the date_default_timezone_set() function. In case you used any of those methods and you are still getting this warning, you most likely misspelled the timezone identifier. We selected the timezone 'UTC' for now, but please set date.timezone to select your timezone. in /home/teknisc/public_html/__biobar.ca/content/catalog.php on line 49

Catalog

12000

HighRanger Plus 100bp DNA Ladder

( 100 loads )
Norgen Biotek

172.06$

Add this item to your cart
retour

Additional description

HighRanger Plus 100 bp DNA Ladder (Cat# 12000 )

DNA Ladder Promotion - Order three or more Norgen DNA Ladders at a special price.


  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise
  • Nineteen discrete fragments ranging from 100bp to 10000bp
  • Higher intensity reference bands at 500bp, 1000bp and 5000bp

Product Description

The Norgen HighRangerPlus 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our HighRangerPlus contains nineteen discrete fragments ranging from 100bp to 10000bp with higher intensity reference bands at 500bp, 1000bp and 5000bp. This Ladder is ideal for size determination of digested DNA.

Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

FEATURES AND BENEFITS

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise



Specifications

Norgen’s DNA Ladders/Markers have been genetically engineered so that the bands are of precise and discrete sizes. Well-spaced bands and higher-intensity reference bands facilitate visual analysis of the gel. These ladders are ideal for quantification due to the precise known ng amounts of each band.Our ladders are ideal for a wide range of applications including size and mass determination of digested DNA and PCR products.

Figure 1: HighRanger Plus 100 bp DNALadder

APPLICATIONS

HighRanger Plus 100 bp DNALadder (Cat# 12000) - 100 loads

Ladder Properties:

  • Nineteen discrete fragments ranging from 100bp to 10000bp.
  • Higher intensity bands at500bp, 1000bp and 5000bp for easy reference.

Fragment
Size (bp)
Mass (ng)
1
10000
42
2
8000
33
3
6000
25
4
5000
42
5
4000
35
6
3000
27
7
2500
22
8
2000
18
9
1500
13
10
1000
40
11
900
28
12 800

25

13 700

34

14 600

19

15 500

44

16 400

13

17 300

18

18 200

13

19 100

10

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.