Catalog

13525

50 bp DNA Ladder

( 100 loads )
Norgen Biotek

172.06$

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Additional description

50 bp DNA Ladder (Cat# 13525 )

DNA Ladder Promotion - Order three or more Norgen DNA Ladders at a special price.


Ideal for PCR product size determination

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise
  • 10 discrete bands from 50 bpto 500 bp
  • Higher intensity reference band at 250 bp

Product Description

The 50 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder containsten discrete fragments ranging from50 bp to500 bp with a higher intensity reference band at 250 bp. This Ladder is ideal for quick sizing of PCR products

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

FEATURES AND BENEFITS

  • Ready-to-use
  • Quantitative
  • Highly Stable
  • Precise

Specifications

Norgen’s DNA Ladders/Markers have been genetically engineered so that the bands are of precise and discrete sizes. Well-spaced bands and higher-intensity reference bands facilitate visual analysis of the gel. These ladders are ideal for quantification due to the precise known ng amounts of each band.Our ladders are ideal for a wide range of applications including size and mass determination of digested DNA and PCR products.

Figure 1: 50 bp DNALadder

APPLICATIONS

50 bp DNALadder (Cat# 13525)- 100 loads

Ladder Properties:
•Ten discrete bands, ranging from 50 bp to 500 bp.
• Higher intensity band at250 bp for easy reference.

Fragment
Size (bp)
Mass (ng)
1
500
79
2
450
67
3
400
59
4
350
55
5
300
49
6
250
76
7
200
33
8
150
22
9
100
31
10
50
29

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:

Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.