Catalog

15002

100b RNA Ladder

( 50 loads )
Norgen Biotek

190.19$

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Additional description

100 b RNA Ladder (Cat# 15002 )

For precise sizing of RNA molecules using native or denaturing gels

  • Eight discrete bands, ranging from 100 to 1,000 bases
  • Higher intensity band at 500 bases for easy reference
  • No need for staining or destaining as loading dye contains ethidium bromide
  • Convenient lyophilized format provides better product stability

Product Description

The Norgen 100b RNA ladder is a set of RNA transcripts derived from recombinant DNA templates. This ladder is suitable for precise sizing of small RNA molecules using native or denaturing agarose gels, and can be easily visualized under UV.

Contents

  • 2 vials of lyophilized RNA ladder (25 loads each).
  • 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution.
  • 2x loading buffer.

FEATURES AND BENEFITS

  • Eight discrete bands, ranging from 100 to 1,000 bases
  • Higher intensity band at 500 bases for easy reference

Specifications

This ladder is ideal for precise sizing of a wide range of RNA molecules using native or denaturing gels.

Figure 1: 100 b RNA Ladder

APPLICATIONS

Specifications:
  • Eight discrete bands, ranging from 100 to 1,000 bases
  • Higher intensity band at 500 bases for easy reference
Contents:
  • 2 vials of lyophilized RNA ladder (25 loads each).
  • 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution.
  • 2x loading buffer.
Instructions:
To reconstitute the lyophilized RNA ladder, add 250 µL of 1x loading buffer to each 25 loads vial and vortex gently.
Heat at 80°C for 10 minutes. Incubate on ice for 1 min. Load 10 µL on a 1.5-2% gel. For optimal results, use Norgen 2x loading buffer with each RNA sample. There is no need for staining and destaining denaturing gels since Norgen’s loading buffer contains ethidium bromide.
Storage:
Store at -20°C.