Catalog

17300

ProteoSpin Abundant Serum Protein Depletion Kit

( 25 preps )
Norgen Biotek

443.76$

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Additional description

Abundant Serum Protein Depletion Kit (Cat# 17300 )

For the rapid depletion of abundant proteins from serum samples

  • Rapid and efficient removal of abundant proteins from serum and plasma samples
  • Convenient and affordable spin column protocol
  • Generic protocol allows for depletion from human and other animal samples

Product Description

The ProteoSpin™ Abundant Serum Depletion Kit provides a fast and simple procedure for the effective depletion of major serum proteins including albumin, α-antitrypsin, transferrin and haptoglobin from serum and plasma samples. This reduces the complexity of the sample and allows for the investigation of less abundant proteins more conveniently. Abundant proteins usually obscure less abundant proteins on gel electrophresis makingit difficult to visualize and recover less abundant proteins. In other applications such mass spectroscopy, fractionating abundant proteins improves the resolution of less abundant proteins. This kit provides a very quick and affordable tool to accomplish the reduction and fractionation of plasma or serum proteins. In addition, since this kit does not employ expensive antibodies that are specific for certain proteins of a specific species, Norgen's kit can be usedto depleteabundant proteins of several species, therefore increasing its versatility and utility.

The kit is unique in that it is based on an ion-exchange mechanism and not the use of specific antibodies. As a result, the kit can be used to deplete serum proteins from a wide variety of samples, including human and various animals.Breifly, the protein sample is bound to the column under specific conditions such that the majority of proteins will bind to the column while the abundant proteins will be removed in the flowthrough (BIND). The bound proteins are then washed to remove any remainingadundant proteins(WASH). Lastly, thedepleted serumproteins are elutedinto a small volume ofthe provided Elution Buffer(ELUTE).Please see theprocedure flowchart to theright.

FEATURES AND BENEFITS

  • Efficient removal of highly abundant proteins - The kit depletes albumin, α-antitrypsin, transferrin and haptoglobin, enabling visualization of low abundance proteins
  • Fast and easy processing - Efficiently process up to 10 samples in less than 30 minutes
  • Does not rely on antibodies- Based on an ion-exchange mechanism, the kit can be applied to serum and plasma samples from a range of sources
  • Complete kit - All columns and solutions required are provided
  • Affordable - Compared to columns based on antibodies, these columns are much lower in price


Download our brochure.

Specifications

Depleted proteins can be used in a number of downstream applications, including:

  • SDS-PAGE
  • 2D gel electrophoresis
  • Mass Spectrometry
  • Protein microarrays
  • DIGE (Difference Gel Electrophoresis)

Figure 1. Depletion of Abundant Proteins. Abundant proteins were depleted from human serum using the ProteoSpinTM Abundant Serum Protein Depletion Kit. Lane 1 represents the input human serum proteins, while Lanes 3 and 4 represent the elution after the abundant proteins were depleted with the kit. Lane 2 represents the binding flowthrough, and contains the proteins that have been depleted from the serum sample.

Figure 2. Depletion of Abundant Proteins. A 2D gel was run after the abundant serum proteins were depleted from human serum using the ProteoSpinTM Abundant Serum Protein Depletion Kit. The abundant proteins including albumin, a-antitrypsin, transferin and haptoglobin have been removed from the sample, allowing the visualization and detection of the lower abundance proteins of interest.

APPLICATIONS

Kit Specifications
Maximum Protein Input
500 µg
Minimum Protein Input
200 µg
Minimum Elution Volume
30 µL
Time to Process 10 Samples
30 minutes


Abundant Serum Protein Depletion Kit Contents

1. Column Activation and Wash Buffer
2. Elution Buffer
3. Neutralizer
4. Mini Spin Columns (25)
5. Elution Tubes (25)
6. Product Insert


Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C.