Catalog

18200

Blood Genomic DNA Isolation Micro

( 50 Preps )
Norgen Biotek

271.70$

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Additional description

Blood DNA Preservation Buffer (3X) (Cat# 29111 )

For rapid and simple preservation of blood DNA at ambient temperatures

  • Preservation of blood DNA for two weeks at room temperature
  • Fast and simple handling, suitable for short-term storage and shipping purposes
  • Compatible with most DNA isolation methods

Product Description

Norgen’s Blood DNA Preservation Buffer (3X)is a 3X aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh blood samples. The buffer prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the buffer eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the buffer allow samples to be stored for one week under conditions where DNA degradation would occur normally. The buffer is intended to be used in clinical laboratories with the ability to preserve samples for use in downstream diagnostic assays.

Storage in the Preservation Buffer

  1. Norgen’s Blood DNA Preservation Buffer (3X)is a 3X solution. One volume of the buffer is added to two volumes of fresh blood followed by mixing by inversion for ten times.
  2. Specimens may be held or shipped to the testing laboratory at ambient temperature for up to 14 days. Specimens held longer should be kept at -20°C or lower until testing.
  3. Storage at -20°C or lower is recommended for archival samples and will provide optimal preservation. The preservation buffer will freeze at -20°C. Samples can be stored indefinitely at -80°C.
  4. Samples can be stored at room temperature (25°C) for up to two week without significant loss of DNA quality.
  5. DNA has also been successfully isolated from samples stored at 37°C for one week.

FEATURES AND BENEFITS

  • No need to immediately process samples - The buffer eliminates the need to immediately process or freeze samples
  • DNA preservation for 2 weeks at room temperature – Intact, biologically active blood DNA has been isolated from samples stored in the Blood DNA Preservation Buffer(3X)for 2 weeks.
  • Ship blood samples at room temperature – Blood samples stored in the Blood DNA Preservation Buffer (3X) can be safely shipped at room temperature with no signs of DNA degradation
  • Compatible with most DNA isolation methods – Blood DNA can be isolated from the preserved samples using a number of different methods, including Norgen’s Blood Genomic DNA Isolation Kits (Cat# 18200, 31000, 31100 & 31200)

Specifications

The preservation buffer is compatible with most DNA and RNA isolation methods. Samples stored in the Blood DNA Preservation Buffer (3X) have been used successfully with all of Norgen Biotek’s blood DNA isolation kits and reagents, including Norgen’s Blood Genomic DNA Isolation Kits (Cat# 18200, 31000, 31100 & 31200).

Figure 1. Integrity of DNA Isolated from Blood Samples Stored Overnight in Heparin and Norgen’s Blood DNA Preservative.One hundredµL of blood collected in a heparin tube (H) and 150 µL of bloodcollectedin Norgen’s Blood DNA Preservation Buffer (3X) (100 µL blood + 50 µL preservative) (N) were stored overnight at 4oC. Blood DNA was then isolated from the samples using Norgen’s RNA/DNA/Protein Purification Kit (Cat #23500), and 20 µL out of each 100 µLelution were loaded on 1% agarose gel.From observing the gel picture above it can be seen that the preserved blood using Norgen's buffer has ahigher DNA yield and integrity.

Figure 2. Stability of DNA Isolated from Preserved Blood Samples Stored up to 2 Weeks at Different Temperatures. A 0.5 mL sample of blood was mixed with 0.25 mL of Norgen’s Blood DNA Preservation Buffer (3X) and stored at different temperatures (-20 °C, 4 °C, room temperature and 37 °C) for 2 weeks. Aliquots of the stored blood were removed at various time points (1 day, 3 days, 8 days and 15 days) and the DNA was isolated using Norgen’s Blood Genomic DNA Isolation Mini Kit (Cat #31000). The purified DNA was then used as the template in a real-time PCR using 5S specific primers. As indicated from the Ct value, all the DNA templates were successfully amplified without significant change in the Ct values over the 15 days.

APPLICATIONS

Norgen’s Blood DNA Preservation Buffer (3X) is available in 2 different sizes:

Size
Catalogue Number
25 mL
29111
100 mL
29112

SHELF LIFE AND HANDLING

  • All solutions should be kept tightly sealed and stored at room temperature (15 – 25 °C) for up to 1 year without any reduction in performance
  • If any precipitation of the preservative is observed in the tubes, heat to 37°C to redissolve