Catalog

18700

Inclusion Body Solubilization Reagent

( 25 mL )
Norgen Biotek

72.45$

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Additional description

Inclusion Body Solubilization Reagent (Cat# 18700 )

For the efficient solubilization of inclusion body proteins

  • Fast dissolving of inclusion body aggregates
  • Allows for downstream applications of inclusion body proteins

Product Description

Norgen's Inclusion Body Solubilization Reagent dissolves inclusion body aggregates resulting from the expression of recombinant proteins in bacteria. This reagent has demonstrated exceptional ablility to solubilize inclusion bodies. Once dissolved, proteins can be analyzed by SDS-PAGE, quantified or further purified for refolding.

Inclusion bodies can be released from bacterial cells using Norgen's Cell Lysis Reagent (available separately). Norgen's Inclusion Body Protein Isolation Kits can also be used as complete kits for cell disruption, inclusion body solubilisation and purification using spin column chromatography

FEATURES AND BENEFITS

Specifications

Solubilized proteins can be used in a number of downstream applications including:

  • SDS-PAGE analysis
  • Western blot
  • ELISA
  • Refolding experiments
  • Mass spectrometry
  • Further purification and scale up



Figure 1: Efficient Isolation of Inclusion Body Proteins Using Norgen Cell Lysis Reagent and Inclusion Body Solubilization Reagent.

Inclusion Bodies were extracted and solubilized following gene expression. Recovered proteins were analyzed on 12% SDS-PAGE and stained with Coomassie Brilliant Blue R-250. Numbers represent kDa sizes of protein bands.

APPLICATIONS